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KMID : 0357920000340110941
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Korean Journal of Pathology
2000 Volume.34 No. 11 p.941 ~ p.949
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Molecular Diagnosis of Cutaneous T Cell Lymphoproliferative Diseases
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Park Ji-Young
Lee Myung-Hoon
Kwak Eun-Kyung
Kim Dong-Ja
Park Tae-In
Bae Han-Ik
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Abstract
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It is often problematic to diagnose T-cell lymphoproliferative disorders of the skin because of the difficulty in establishing clonality in paraffin-embedded tissue. We used polymerase chain reaction single strand conformational polymorphism (PCR-SSCP) and heteroduplex analysis in paraffin embedded tissue to detect clonal rearrangement of T-cell receptor γ (TCRγ) gene in 17 T-cell lymphoproliferative disorders and 6 atypical lymphoproliferative diseases. We used polymerase chain reaction to detect TCRβ gene rearrangement in 8 of 17 cases which did not show TCRγ gene rearrangement. Jurkat cell lines were used as monoclonal controls. DNA was extracted from 5 biopsies of T-cell lymphomas, 10 biopsies of mycosis fungoides, 2 biopsies of lymphomatoid papulosis, and 6 biopsies of atypical lymphoproliferative lesions. We detected monoclonality in 5 of 5 T-cell lymphoma cases, 2 of 2 lymphomatoid papulosis cases, 6 of 10 mycosis fungoides cases, and 2 of 6 atypical lymphoproliferative disease cases. We conclude that nonradioactive PCR-SSCP for TCR gene rearrangement analysis is a useful adjunct to routine histological and immunophenotypic methods in the diagnosis of cutaneous T cell lymphoproliferative disorders in paraffin embedded tissue.
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KEYWORD
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Polymerase chain reaction, Single strand conformational polymorphism, Heteroduplex, T-cell receptor, Cutaneous T-cell lymphoproliferative diseases
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